Journal: Science Advances

Article Title: Small-molecule inhibitor targeting the Hsp90-Cdc37 protein-protein interaction in colorectal cancer

doi: 10.1126/sciadv.aax2277

Figure Lengend Snippet: ( A ) Co-IP in HCT116 cells after treatment with DMSO and increasing concentrations of DDO-5936 (5, 10, and 25 μM) for 24 hours. Western blots were performed with anti-Hsp90, anti-Cdc37, or anti-CDK4 in each experiment. Data are representative of three independent experiments. ( B ) Correlation between the protein expression level of Hsp90 and Cdc37 in diverse cell lines and antiproliferative activities (IC 50 values). The Pearson correlation coefficient ( r ) was calculated by GraphPad Prism 6.0 software. Data are presented as the means ± SD, n = 6 wells, from three independent experiments. ( C ) Western blot analysis of Cdc37, p (phosphorylated)–Cdc37, Hsp90, Hsp70, GR (glucocorticoid receptor), p-GR, AKT, p-AKT, ERK1/2 (extracellular signal–regulated kinase 1/2), p-ERK1/2, CDK4, and CDK6 protein expression levels in HCT116 cells after treatment with 0, 1, 5, 10, 20, and 40 μM DDO-5936 or 0, 0.5, 1, and 5 μM AT13387 for 24 hours. β-Actin was used as a loading control. Data are representative of three independent experiments.

Article Snippet: The IC 50 values were calculated by nonlinear fit curves using GraphPad Prism 6.0 software.

Techniques: Co-Immunoprecipitation Assay, Western Blot, Expressing, Software